IL12RB1 antibody (Biotin)

Catalog No. ABIN2689093

Cited in 8+ publications. The Biotin-conjugated Mouse Monoclonal anti-IL12RB1 antibody (Clone 114) (ABIN2689093) specifically detects IL12RB1 in FACS. The antibody is reactive with Mouse samples.

Quick Overview for IL12RB1 antibody (Biotin) (ABIN2689093)

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12RB1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 114

Product Details anti-IL12RB1 Antibody (Biotin)

Characteristics: The 114 antibody reacts with mouse CD212 (the β1 subunit of IL-12Rβ1), originally termed IL-12Rβ, of the mouse IL-12 receptor complex. The IL-12Rβ1 subunit associates with a β2 subunit to form a heterodimeric IL-12 receptor complex. Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity. The IL-12Rβ1 subunit interacts primarily with IL-12 p40 whereas the IL-12Rβ2 binds both to IL-12 p40 and IL-12 p35. IL-12Rβ1 is required for high affinity binding of IL-12 but IL-12Rβ2 is required for signaling. IL-12Rβ1 has more recently been described to bind IL-23, a heterodimer formed of the p40 subunit from IL-12, and p19. The cytoplasmic regions of the β1 and β2 subunits contain the box1 and box2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR. IL-12Rβ1 are primarily expressed by activated T cells and NK cells. Experiments with IL-12Rβ1 deficient mice have shown that IL-12Rβ1 is necessary for mouse T and NK cell responsiveness to IL-12 p75. The 114 antibody was generated by immunizing IL-12Rβ1 deficient mice of (129 x BALB/c)F1 background with mouse Ba/F3 cells that were stably transfected with IL-12Rβ1.

BD Pharmingen™ Biotin Mouse Anti-Mouse CD212 - Biotin - Clone 114 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: IL-12Rbeta1 Transfected Cell Line

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL12RB1 antibody (Biotin) (ABIN2689093)

Wu, Wang, Gadina, OShea, Presky, Magram: "IL-12 receptor beta 2 (IL-12R beta 2)-deficient mice are defective in IL-12-mediated signaling despite the presence of high affinity IL-12 binding sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6221-8, (2000) (PubMed).

Wang, Wilkinson, Podlaski, Wu, Stern, Presky, Magram: "Characterization of mouse interleukin-12 p40 homodimer binding to the interleukin-12 receptor subunits." in: European journal of immunology, Vol. 29, Issue 6, pp. 2007-13, (1999) (PubMed).

Gately, Renzetti, Magram, Stern, Adorini, Gubler, Presky: "The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses." in: Annual review of immunology, Vol. 16, pp. 495-521, (1998) (PubMed).

Presky, Minetti, Gillessen, Wilkinson, Wu, Gubler, Chizzonite, Gately: "Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2174-9, (1998) (PubMed).

Wu, Ferrante, Gately, Magram: "Characterization of IL-12 receptor beta1 chain (IL-12Rbeta1)-deficient mice: IL-12Rbeta1 is an essential component of the functional mouse IL-12 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1658-65, (1997) (PubMed).

Presky, Yang, Minetti, Chua, Nabavi, Wu, Gately, Gubler: "A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 14002-7, (1997) (PubMed).

Chua, Wilkinson, Presky, Gubler: "Cloning and characterization of a mouse IL-12 receptor-beta component." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 9, pp. 4286-94, (1995) (PubMed).

Stahl, Yancopoulos: "The alphas, betas, and kinases of cytokine receptor complexes." in: Cell, Vol. 74, Issue 4, pp. 587-90, (1993) (PubMed).

Target Details for IL12RB1

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Alternative Name: CD212

Background: Synonyms: IL-12 Receptor β1 chain

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation