ICAM1 antibody

Catalog No. ABIN2689333

Cited in 8+ publications. The Mouse Monoclonal anti-ICAM1 antibody (Clone LB) (ABIN2689333) specifically detects ICAM1 in FACS, IHC, IP and Func. The antibody is reactive with Human samples.

Quick Overview for ICAM1 antibody (ABIN2689333)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func)

Clone: LB

Product Details anti-ICAM1 Antibody

Characteristics: Anti-ICAM-1 (CD54) monoclonal antibody (clone LB-2) recognizes an inducible 76- to 110 kD glycoprotein, intercellular adhesion molecule-1 (ICAM-1), the CD54 antigen, that is expressed on human myeloma cells, Burkitt's lymphoma cells, erythroleukemia cells, B-lymphoblastoid cells, monocytes, and granulocytes, but not on T-cell lines, resting T lymphocytes or T-cell leukemias.

BD Pharmingen™ Purified Mouse Anti-Human CD54 - Purified - Clone LB-2 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.2 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Major histocompatibility Class II antigen negative variant of human B-lymphoblastoid cell line

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ICAM1 antibody (ABIN2689333)

Lo, Lee, Ramos, Lobb, Rosa, Chi-Rosso, Wright: "Endothelial-leukocyte adhesion molecule 1 stimulates the adhesive activity of leukocyte integrin CR3 (CD11b/CD18, Mac-1, alpha m beta 2) on human neutrophils." in: The Journal of experimental medicine, Vol. 173, Issue 6, pp. 1493-500, (1991) (PubMed).

Diamond, Staunton, de Fougerolles, Stacker, Garcia-Aguilar, Hibbs, Springer: "ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18)." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 2, pp. 3129-39, (1991) (PubMed).

Phillips, Nudelman, Gaeta, Perez, Singhal, Hakomori, Paulson: "ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex." in: Science (New York, N.Y.), Vol. 250, Issue 4984, pp. 1130-2, (1991) (PubMed).

Bevilacqua, Stengelin, Gimbrone, Seed: "Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins." in: Science (New York, N.Y.), Vol. 243, Issue 4895, pp. 1160-5, (1989) (PubMed).

Bevilacqua, Pober, Mendrick, Cotran, Gimbrone: "Identification of an inducible endothelial-leukocyte adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 24, pp. 9238-42, (1988) (PubMed).

Makgoba, Sanders, Ginther Luce, Dustin, Springer, Clark, Mannoni, Shaw: "ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells." in: Nature, Vol. 331, Issue 6151, pp. 86-8, (1988) (PubMed).

Patarroyo, Clark, Prieto, Kantor, Gahmberg: "Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB-2." in: FEBS letters, Vol. 210, Issue 2, pp. 127-31, (1987) (PubMed).

Clark, Ledbetter, Holly, Dinndorf, Shu: "Polypeptides on human B lymphocytes associated with cell activation." in: Human immunology, Vol. 16, Issue 1, pp. 100-13, (1986) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: Synonyms: Human ICAM-1, HICAM-1

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling