CD81 antibody (Biotin)

Catalog No. ABIN2689414

Product Details anti-CD81 Antibody (Biotin)

Target: CD81

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD81 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The Eat2 antibody reacts with CD81, a 26- kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with αβ T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen. Two-color analysis of the expression of CD81 on mouse and rat splenocytes. C57BL/6 splenocytes were simultaneously stained with FITC Rat anti-Mouse CD45R/B220 (Cat. no. 553087/553088, First and second panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, First panel) or PE Hamster Anti-Mouse/Rat CD81 (Cat. No, 559519, Second panel) monoclonal antibodies. Similarly, LOU splenocytes were simultaneously stained with FITC Mouse anti-Rat CD45RA (Cat. No. 554883, Third and last panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, Third panel) or PE Hamster anti-Mouse/Rat CD81 (Cat. No, 559519, Last panel) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ Flow Cytometry System (BD Biosciences,San Jose, CA

BD Pharmingen™ Biotin Hamster Anti-Mouse/Rat CD81 - Biotin - Clone Eat2 - Isotype Armenian Hamster IgG, κ - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CD81+ mouse B lymphoma 38C13

Clone: Eat2

Isotype: IgG kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD81 antibody (Biotin) (ABIN2689414)

Deng, Yeung, Tsitoura, DeKruyff, Umetsu, Levy: "Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5054-61, (2000) (PubMed).

Maecker, Todd, Kim, Levy: "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." in: Hybridoma, Vol. 19, Issue 1, pp. 15-22, (2000) (PubMed).

Maecker, Do, Levy: "CD81 on B cells promotes interleukin 4 secretion and antibody production during T helper type 2 immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2458-62, (1998) (PubMed).

Levy, Todd, Maecker: "CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system." in: Annual review of immunology, Vol. 16, pp. 89-109, (1998) (PubMed).

Fleming, Donnadieu, Song, Laethem, Galli, Kinet: "Negative regulation of Fc epsilon RI-mediated degranulation by CD81." in: The Journal of experimental medicine, Vol. 186, Issue 8, pp. 1307-14, (1997) (PubMed).

Maecker, Levy: "Normal lymphocyte development but delayed humoral immune response in CD81-null mice." in: The Journal of experimental medicine, Vol. 185, Issue 8, pp. 1505-10, (1997) (PubMed).

Miyazaki, Müller, Campbell: "Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81." in: The EMBO journal, Vol. 16, Issue 14, pp. 4217-25, (1997) (PubMed).

Tsitsikov, Gutierrez-Ramos, Geha: "Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 20, pp. 10844-9, (1997) (PubMed).

Boismenu, Rhein, Fischer, Havran: "A role for CD81 in early T cell development." in: Science (New York, N.Y.), Vol. 271, Issue 5246, pp. 198-200, (1996) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 (CD81 Products)

Synonyms: Tapa-1 antibody, Tapa1 antibody, Tspan28 antibody, CVID6 antibody, S5.7 antibody, TAPA1 antibody, TSPAN28 antibody, CD81 molecule antibody, CD81 molecule b antibody, CD81 antigen antibody, Cd81 molecule antibody, CD81 antibody, cd81b antibody, Cd81 antibody

Background: Synonyms: TAPA-1, Tspan28, Tapa1, Tapa-1, Target of the antiproliferative antibody 1,

Pathways: Inositol Metabolic Process, Hepatitis C