CD86 antibody
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- Target See all CD86 Antibodies
- CD86
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD86 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- BD Pharmingen™
- Characteristics
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The 24F antibody reacts with CD86 (B7-2), a member of the Ig superfamily of transmembrane proteins. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD86 is predominantly expressed on antigen-presenting cells. It can be upregulated on splenic B cells by in vitro polyclonal activation, such as LPS treatment. 24F mAb is reported to block the costimulatory function of rat CD86 and to immunoprecipitate CD86 from Lewis-S1 cell lysates. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Mouse Anti-Rat CD86 - Purified - Clone 24F - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- HTLV-1-transformed rat T-cell line Lewis-S1
- Clone
- 24F
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD86 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
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Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
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- Target
- CD86
- Alternative Name
- CD86 (CD86 Products)
- Synonyms
- B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
- Background
- Synonyms: B7-2
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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