IL-1 beta antibody

Catalog No. ABIN2689747

Cited in 2+ publications. The Armenian Hamster Monoclonal anti-IL-1 beta antibody (Clone B122) (ABIN2689747) specifically detects IL-1 beta in WB, IP, ELISA and Neut. The antibody is reactive with Mouse samples.

Quick Overview for IL-1 beta antibody (ABIN2689747)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Neutralization (Neut)

Clone: B122

Product Details anti-IL-1 beta Antibody

Characteristics: The B122 antibody recognizes the precursor and mature secreted forms of the mouse IL-1β protein. B122 neutralizes the biological activity of mouse IL-1β, but not the biological activities of mouse IL-1α nor human IL-1β. B122 does not recognize mouse IL-2, IL-4, IL-6, IL-10, TNF or IFN-γ. This antibody also recognizes rat IL-1β. The immunogen used to generate the B122 hybridoma was a purified, mature form of the mouse IL-1β protein.

BD Pharmingen™ Purified Hamster Anti-Mouse IL-1β - Purified - Clone B122 - Isotype Armenian Hamster IgG, λ1 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse IL-1beta

Isotype: IgG lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-1 beta antibody (ABIN2689747)

Miller, Krasney, Gauvin, Holbrook, Koonz, Abruzzese, Miller, Pagani, Dolle, Ator: "Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 3, pp. 1331-8, (1995) (PubMed).

Hogquist, Nett, Sheehan, Pendleton, Schreiber, Chaplin: "Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 5, pp. 1534-40, (1991) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome