IL-6 antibody

Catalog No. ABIN2689776

There are 4+ publications for this product available. The Rat Monoclonal anti-IL-6 antibody is suitable to detect IL-6 in samples from Human. It has been validated for WB, ELISA, Neut and Inhibition.

Quick Overview for IL-6 antibody (ABIN2689776)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: MQ2

Product Details anti-IL-6 Antibody

Characteristics: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate this hybridoma was COS-7 -expressed recombinant human IL-6. This is a neutralizing antibody. This antibody is routinely tested by ELISA. Other applications were tested during antibody development only or reported in the literature. ELISA standard curve for human IL-6.

BD Pharmingen™ Purified Rat Anti-Human IL-6 - Purified - Clone MQ2-13A5 - Isotype Rat IgG1 - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-6 antibody (ABIN2689776)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Gaines Das, Poole: "The international standard for interleukin-6. Evaluation in an international collaborative study." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 147-53, (1993) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome