IL-6 antibody

Catalog No. ABIN2689777

Cited in 4+ publications. The Rat Monoclonal anti-IL-6 antibody (Clone MP5) (ABIN2689777) specifically detects IL-6 in WB, ELISA, Neut and Inhibition. The antibody is reactive with Mouse samples.

Quick Overview for IL-6 antibody (ABIN2689777)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: MP5

Product Details anti-IL-6 Antibody

Characteristics: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The immunogen used to generate the MP5-20F3 hybridoma was recombinant mouse IL-6. This is a neutralizing antibody.

BD Pharmingen™ Purified Rat Anti-Mouse IL-6 - Purified - Clone MP5-20F3 - Isotype Rat IgG1 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-6 antibody (ABIN2689777)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Suda, OGarra, MacNeil, Fischer, Bond, Zlotnik: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, (1990) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome