CD14 antibody

Catalog No. ABIN2689901

This Rat Monoclonal antibody specifically detects CD14 in ELISA. It exhibits reactivity toward Human. It has been mentioned in 9+ publications

Quick Overview for CD14 antibody (ABIN2689901)

Target: CD14

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: ELISA

Clone: 55

Product Details anti-CD14 Antibody

Characteristics: The 55-3 monoclonal antibody reacts with human CD14. CD14 is a 53 - 55 kDa cell surface glycoprotein that is expressed by monocytes, macrophages, and activated granulocytes as a glycophosphatidylinositol-linked protein. CD14 functions as a receptor for lipopolysaccharide (LPS, endotoxin) when LPS is bound to LBP (LPS binding protein). Soluble forms of CD14 (sCD14) can be detected either in serum or in supernatants from cell cultures. Soluble CD14 is produced either by shedding of membrane CD14 or by release from cells before addition of the GPI anchor. Both membrane and sCD14 function to enhance cell activation by LPS. Binding of LPS to CD14 activates monocytes or macrophages to produce cytokines and express adhesive proteins and low molecular weight proinflammatory mediators. Non-CD14 expressing cells, such as endothelial cells, can be activated by a complex of LPS and sCD14. Serum levels of sCD14 increase in association with sepsis, infectious disease, autoimmunity, and allergic disorders. The gene for human CD14 has been cloned and sequenced and recombinant sCD14 has been shown to be protective against LPS-induced lethality. The immunogen used to generate the 55-3 hybridoma was purified, soluble recombinant human CD14 protein.

BD Pharmingen™ Purified Rat Anti-Human sCD14 - Purified - Clone 55-3 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD14 Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD14 antibody (ABIN2689901)

Egerer, Feist, Rohr, Pruss, Burmester, Dörner: "Increased serum soluble CD14, ICAM-1 and E-selectin correlate with disease activity and prognosis in systemic lupus erythematosus." in: Lupus, Vol. 9, Issue 8, pp. 614-21, (2000) (PubMed).

Nockher, Wick, Pfister: "Cerebrospinal fluid levels of soluble CD14 in inflammatory and non-inflammatory diseases of the CNS: upregulation during bacterial infections and viral meningitis." in: Journal of neuroimmunology, Vol. 101, Issue 2, pp. 161-9, (1999) (PubMed).

Haziot, Rong, Lin, Silver, Goyert: "Recombinant soluble CD14 prevents mortality in mice treated with endotoxin (lipopolysaccharide)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 12, pp. 6529-32, (1995) (PubMed).

Ulevitch, Tobias: "Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin." in: Annual review of immunology, Vol. 13, pp. 437-57, (1995) (PubMed).

Arditi, Zhou, Dorio, Rong, Goyert, Kim: "Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14." in: Infection and immunity, Vol. 61, Issue 8, pp. 3149-56, (1993) (PubMed).

Haziot, Rong, Silver, Goyert: "Recombinant soluble CD14 mediates the activation of endothelial cells by lipopolysaccharide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1500-7, (1993) (PubMed).

Ferrero, Goyert: "Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14." in: Nucleic acids research, Vol. 16, Issue 9, pp. 4173, (1988) (PubMed).

Haziot, Chen, Ferrero, Low, Silber, Goyert: "The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 547-52, (1988) (PubMed).

Goyert, Ferrero, Rettig, Yenamandra, Obata, Le Beau: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." in: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, (1988) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: sCD14

Background: Synonyms: Soluble CD14

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades