TCR gamma/delta antibody

Catalog No. ABIN2689920

Product Details anti-TCR gamma/delta Antibody

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: Recognizes the γ/δ T-cell receptor (TCR). This receptor complex consists of two disulfide-linked glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). γ/δ TCR is expressed in less than 10 % of human peripheral T cells. The physiological significance of γ/δ+ T cells is still unknown. There is evidence indicating that these cells recognize bacterial ligands and some tumor cells. Reports suggest that γ/δ+ T cells may play a role in the immune reaction during infection and in regulation of pathphysiological autoimmune responses. Due to the fact that CD3 and TCRγδ antibodies may recognize epitopes which are very close to each other and that CD3 was interfering with the clone B1 antibody binding but not with the clone 11F2 antibody, we recommend to use clone 11F2 antibody to perform double staining TCRγδ and CD3. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Purified Mouse Anti-Human TCR γδ - Purified - Clone B1 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: B1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TCR gamma/delta antibody (ABIN2689920)

Kabelitz: "Function and specificity of human gamma/delta-positive T cells." in: Critical reviews in immunology, Vol. 11, Issue 5, pp. 281-303, (1992) (PubMed).

Kabelitz, Pechhold, Bender, Wesselborg, Wesch, Friese, Janssen: "Activation and activation-driven death of human gamma/delta T cells." in: Immunological reviews, Vol. 120, pp. 71-88, (1991) (PubMed).

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: TCR Gammadelta (TCR gamma/delta Products)