TNF alpha antibody (Biotin)

Catalog No. ABIN2689928

This Human Monoclonal antibody specifically detects TNF alpha in ELISA. It exhibits reactivity toward Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for TNF alpha antibody (Biotin) (ABIN2689928)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse, Rat

Host: Human

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA

Clone: 516D1A1

Product Details anti-TNF alpha Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The 516D1A1 Fab antibody reacts with mouse and rat tumor-necrosis factor (TNF, formerly known as TNF-α) proteins. The 516D1A1 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with recombinant mouse TNF protein. The biology and function of the TNF protein has been extensively reviewed (Hehlgans et al.) in the literature. The biotinylated 516D1A1 antibody is useful as a detection antibody for a sandwich ELISA that measures mouse and rat TNF protein levels. Purified Hamster Anti-Mouse/Rat TNF (Cat. No.557516) as capture antibody can be paired with biotinylated 516D1A1 as the detection antibody, using purified recombinant mouse TNF protein (Cat. No. 554589) or purified recombinant rat TNF protein (Cat. No. 555109) as standard. The biotinylated 516D1A1 antibody should be titrated between 0.25 - 2.0 μg/mL to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse or rat TNF protein ranging from 31.25 pg/mL to 2,000 pg/mL are recommended for inclusion in each ELISA plate. Investigators may find methodology information to be useful in Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences (2003)-- Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. For this ELISA antibody pair, no cross-reactivity has been observed with the following: Rat IL-1α, IL-2, IL-4, IL-6, IL-10, IL-18, GM-CSF, IFN-γ, mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-18, M-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, sTNFRI, sTNFRII, RANTES, human TNF. Mouse TNF sandwich ELISA curve: The curve was generated by sandwich ELISA using the purified TN3-19.12 (Cat. No. 557516) as capture antibody, doubling dilutions of recombinant mouse TNF protein (Cat. No. 554589) and biotin 516D1A1 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.

BD Pharmingen™ Biotin Human anti-Mouse/Rat TNF - Biotin - Clone 516D1A1 - Isotype Human Ig, κ - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse TNF

Isotype: Ig Fraction, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TNF alpha antibody (Biotin) (ABIN2689928)

Hehlgans, Pfeffer: "The intriguing biology of the tumour necrosis factor/tumour necrosis factor receptor superfamily: players, rules and the games." in: Immunology, Vol. 115, Issue 1, pp. 1-20, (2005) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome