CDKN2B antibody

Catalog No. ABIN271760

The Rabbit Polyclonal anti-CDKN2B antibody is suitable to detect CDKN2B in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for CDKN2B antibody (ABIN271760)

Target: CDKN2B (Cyclin-Dependent Kinase Inhibitor 2B (p15, Inhibits CDK4) (CDKN2B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDKN2B Antibody

Specificity: This antibody detects endogenous levels of p15 INK protein. (region surrounding Gly113)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDKN2B

Target: CDKN2B (Cyclin-Dependent Kinase Inhibitor 2B (p15, Inhibits CDK4) (CDKN2B))

Alternative Name: CDKN2B / p15INK4b

Background: The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFβ-treated human keratinocytes, suggesting that p15 may act as an effector of TGFβ-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFβ-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.Synonyms: Cyclin-dependent kinase 4 inhibitor B, MTS2, Multiple tumor suppressor 2, p14-INK4b, p14INK4b, p15-INK4b

Molecular Weight: approx. 15 kDa

Gene ID: 1030

NCBI Accession: NP_004927

UniProt: P42772

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases