PML antibody

Catalog No. ABIN271787

The Rabbit Polyclonal anti-PML antibody is suitable to detect PML in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for PML antibody (ABIN271787)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PML Antibody

Specificity: This antibody detects endogenous levels of PML protein. (region surrounding Pro39)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity-chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML / RNF71

Background: The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15,17) (q22,q11.2-q12) chromosomal trans-location of the retinoic acid receptor alpha (RARalpha) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARalpha chimeric protein. Myeloid precursor cells expressing the PML/ RARalpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARalpha transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARalpha also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.Synonyms: MYL, Probable transcription factor PML, RING finger protein 71, TRIM19, Tripartite motif-containing protein 19

Molecular Weight: approx. 98 kDa

Gene ID: 5371

NCBI Accession: NP_002666

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus