Collagen Type I antibody

Catalog No. ABIN272015

The Rabbit Polyclonal anti-Collagen Type I antibody has been validated for IF, IHC (p) and EIA. It is suitable to detect Collagen Type I in samples from Human, Rat and Mouse.

Quick Overview for Collagen Type I antibody (ABIN272015)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody detects endogenous levels of Collagen I protein. (region surrounding Ser3)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. Immunoflourescence: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % sodium azide

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.Synonyms: Alpha-2 type I collagen, COL1A2

Molecular Weight: approx. 125 kDa

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452