UBTF antibody

Catalog No. ABIN272172

The Rabbit Polyclonal anti-UBTF antibody (ABIN272172) specifically detects UBTF in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBTF antibody (ABIN272172)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBTF Antibody

Specificity: This antibody detects endogenous levels of UBF-1 protein. (region surrounding Glu536)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBTF / UBF

Background: The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the 70 kDa and 86 kDa Ku antigens.Synonyms: Autoantigen NOR-90, Nucleolar transcription factor 1, UBF-1, UBF1, Upstream-binding factor 1

Molecular Weight: approx. 90 kDa

Gene ID: 7343

NCBI Accession: NP_001070151

UniProt: P17480