TP53I3 antibody

Catalog No. ABIN272242

This Rabbit Polyclonal antibody specifically detects TP53I3 in WB. It exhibits reactivity toward Human.

Quick Overview for TP53I3 antibody (ABIN272242)

Target: TP53I3 (Tumor Protein P53 Inducible Protein 3 (TP53I3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53I3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TP53I3 Antibody

Specificity: This antibody detects endogenous levels of PIG3 protein. (region surrounding Leu265)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for TP53I3

Target: TP53I3 (Tumor Protein P53 Inducible Protein 3 (TP53I3))

Alternative Name: TP53I3

Background: The PIG (p53-induced gene) gene family encodes redox-controlling proteins that are involved in p53 tumor suppressor activity. One member of the PIG gene family, p53-inducible gene 3 (PIG3), is a p53 responsive gene that maps, in humans, to chromosome 2p and encodes a protein with significant homology to oxidoreductases. Oxidoreductases are enzymes involved in cellular responses to oxidative stress and irradiation, and they influence the involvement of PIG3 in the metabolism of reactive oxygen species. PIG3 is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 is p53 dependent and occurs with delayed kinetics as compared with other p53 downstream targets. PIG3 may act with caspase-8 as a key regulatory element in p53-dependent transcriptional deregulation by triggering the caspase cascade and mitochondrial breakdown. PIG3 is highly up-regulated by p53 and may be useful for detecting transient activation of p53.Synonyms: PIG3, Quinone oxidoreductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein

Molecular Weight: approx. 36 kDa

Gene ID: 9540

NCBI Accession: NP_001193731

UniProt: Q53FA7

Pathways: p53 Signaling