ERAP1 antibody

Catalog No. ABIN272255

Product Details anti-ERAP1 Antibody

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of ERAP1 protein. (region surrounding Lys467)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry (Paraffin): 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ERAP1

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Alternative Name: ERAP1 / ARTS1 (ERAP1 Products)

Synonyms: MGC166160 antibody, DKFZp469C044 antibody, ARTS-1 antibody, A-LAP antibody, ALAP antibody, APPILS antibody, ARTS1 antibody, ERAAP antibody, ERAAP1 antibody, PILS-AP antibody, PILSAP antibody, Arts1 antibody, PILSA antibody, Appils antibody, endoplasmic reticulum aminopeptidase 1 antibody, endoplasmic reticulum aminopeptidase 1b antibody, ERAP1 antibody, erap1 antibody, erap1b antibody, Erap1 antibody

Background: The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon-γ. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.Synonyms: APPILS, ARTS-1, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, Endoplasmic reticulum aminopeptidase 1, KIAA0525, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Molecular Weight: approx. 110 kDa

Gene ID: 51752

NCBI Accession: NP_001035548

UniProt: Q9NZ08