Insulin antibody (AA 1-84)

Catalog No. ABIN2742155

The Mouse Monoclonal anti-Insulin antibody has been validated for IHC, WB, ELISA, IF, IHC (p), FACS and IP. It is suitable to detect Insulin in samples from Human, Pig and Cow.

Quick Overview for Insulin antibody (AA 1-84) (ABIN2742155)

Target: Insulin (INS)

Reactivity: Human, Pig, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 2D11-H5 or INS05

Product Details anti-Insulin Antibody

Binding Specificity: AA 1-84

Specificity: Pig Insulin

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G purified

Immunogen: Full length purified pig Insulin (aa1-84).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. antibody to insulin is an important beta-cell and insulinoma marker. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gene ID: 3630

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation