Poliovirus Receptor antibody (Biotin)

Catalog No. ABIN2743906

This Mouse Monoclonal antibody specifically detects Poliovirus Receptor in ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Poliovirus Receptor antibody (Biotin) (ABIN2743906)

Target: Poliovirus Receptor (PVR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Poliovirus Receptor antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: ANC2B2

Product Details anti-Poliovirus Receptor Antibody (Biotin)

Specificity: Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

Purification: Protein A purified

Immunogen: Recombinant Human CD155.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 μL of 300 ^g/mL human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD155/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for Poliovirus Receptor

Target: Poliovirus Receptor (PVR)

Alternative Name: PVR / CD155

Background: Name/Gene ID: PVR

Synonyms: PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4

Gene ID: 5817

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome