BCMA antibody (PE)

Catalog No. ABIN2744987

The Mouse Monoclonal anti-BCMA antibody is suitable to detect BCMA in samples from Human. It has been validated for ELISA and FACS.

Quick Overview for BCMA antibody (PE) (ABIN2744987)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCMA antibody is conjugated to PE

Application: ELISA, Flow Cytometry (FACS)

Clone: ANC3B1

Product Details anti-BCMA Antibody (PE)

Specificity: Antibody ANC3B1 binds to recombinant CD269 (but not CD268) in EIA and native CD269 expressed on Raji cell surface. It blocks binding of recombinant soluble CD257(BAFF)trn-muCD8 to recombinant CD269(BCMA)-muIg in EIA.

Purification: Protein A purified

Immunogen: Recombinant human CD269.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 cultured Raji cells were washed and pre incubated ~5 minutes with 20 μL of 250 μg/mL human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD269/R-PE at a dilution factor of 1:50 (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.51 log10 fluorescent units when compared to a Mouse IgG1/RPE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD269 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for BCMA

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: TNFRSF17 / BCMA

Background: Name/Gene ID: TNFRSF17
Family: TNF Receptor

Synonyms: TNFRSF17, B cell maturation antigen, BCMA, CD269 antigen, B-cell maturation factor, B-cell maturation protein, BCM, CD269

Gene ID: 608