BCMA antibody (Biotin)

Catalog No. ABIN2744988

The Biotin-conjugated Mouse Monoclonal anti-BCMA antibody (Clone ANC3B1) (ABIN2744988) specifically detects BCMA in ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for BCMA antibody (Biotin) (ABIN2744988)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCMA antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: ANC3B1

Product Details anti-BCMA Antibody (Biotin)

Specificity: Antibody ANC3B1 binds to recombinant CD269 (but not CD268) in EIA and native CD269 expressed on Raji cell surface. It blocks binding of recombinant soluble CD257(BAFF)trn-muCD8 to recombinant CD269(BCMA)-muIg in EIA.

Purification: Protein A purified

Immunogen: Recombinant human CD269.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ill of anti-CD269/Biotin at a concentration of 10 ilg/mL. Cells were washed twice and incubated with 2o reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.58 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cell s were pre incubated 10 minutes with anti-CD269 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for BCMA

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: TNFRSF17 / BCMA

Background: Name/Gene ID: TNFRSF17
Family: TNF Receptor

Synonyms: TNFRSF17, B cell maturation antigen, BCMA, CD269 antigen, B-cell maturation factor, B-cell maturation protein, BCM, CD269

Gene ID: 608