TNF alpha antibody

Catalog No. ABIN2749097

This Mouse Monoclonal antibody specifically detects TNF alpha in ELISA, ICC, IHC (fro) and ICFC. It exhibits reactivity toward Human, Pig and Non-Human Primate and has been mentioned in 4+ publications.

Quick Overview for TNF alpha antibody (ABIN2749097)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: MAb11

Product Details anti-TNF alpha Antibody

Purpose: Anti-TNF alpha Purified

Specificity: The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Cross-Reactivity (Details): Human, Non-Human Primates, Porcine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant human TNF-alpha

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNF alpha antibody (ABIN2749097)

Yan, Qing, Byers, Stadnyk, Al-Hertani, Bortolussi: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." in: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, (2004) (PubMed).

Attarbaschi, Willheim, Ramharter, Hofmann, Wahl, Winkler, Graninger, Winkler: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." in: European cytokine network, Vol. 14, Issue 1, pp. 34-9, (2003) (PubMed).

Visser, Graffelman, Blauw, Haspels, Lentjes, de Kloet, Nagelkerken: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." in: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, (2001) (PubMed).

Wahlström, Katchar, Wigzell, Olerup, Eklund, Grunewald: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." in: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, (2001) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Tumor necrosis factor,TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.,TNFA, DIF, TNLG1F

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome