ARHGEF4 antibody (AA 143-271) (PE)

Catalog No. ABIN2749098

The Mouse Monoclonal anti-ARHGEF4 antibody is suitable to detect ARHGEF4 in samples from Human. It has been validated for ICFC.

Quick Overview for ARHGEF4 antibody (AA 143-271) (PE) (ABIN2749098)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARHGEF4 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: ARHGEF-08

Product Details anti-ARHGEF4 Antibody (PE)

Binding Specificity: AA 143-271

Purpose: Anti-ARHGEF4 PE

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4

Background: Rho guanine nucleotide exchange factor 4,ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –, stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.,ASEF1, GEF4, STM6

Gene ID: 50649

UniProt: Q9NR80

Pathways: Neurotrophin Signaling Pathway