ERCC1 antibody

Catalog No. ABIN2763838

This Rabbit Polyclonal antibody specifically detects ERCC1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ERCC1 antibody (ABIN2763838)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ERCC1 Antibody

Cross-Reactivity: Human

Characteristics: Excision Repair Cross Complementing protein-1, Nucleotide Excision Repair Protein,Recognizes a protein of 33-36 kDa, identified as the Excision Repair Cross Complementing (ERCC1). The mammalian ERCC1-encoded polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts, and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.

Purification: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide derived from human ERCC1 protein

Isotype: Ig Fraction

Application Details

Application Notes: This antibody can be used for Western blotting (5-10 μg/mL) and immunohistochemistry on formalin/paraffin sections (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (DNA excision repair protein)

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response