H2AFX antibody (pSer140)

Catalog No. ABIN2763904

This Rabbit Polyclonal antibody specifically detects H2AFX in WB, IHC and EIA. It exhibits reactivity toward Human.

Quick Overview for H2AFX antibody (pSer140) (ABIN2763904)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Product Details anti-H2AFX Antibody

Binding Specificity: pSer140

Cross-Reactivity: Human

Characteristics: H2AFX, H2AX,Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Purification: Antigen Immunoaffiinity Purification

Immunogen: Synthetic peptide derived from human histone H2a.x protein. Sequence is unphosphorylated version of peptide.

Isotype: IgG

Application Details

Application Notes: Antibody can be used for immunohistochemical staining (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2A.x

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus