SPHK1 antibody (pSer225)

Catalog No. ABIN2764174

This Rabbit Polyclonal antibody specifically detects SPHK1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for SPHK1 antibody (pSer225) (ABIN2764174)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SPHK1 Antibody

Binding Specificity: pSer225

Cross-Reactivity: Mouse

Characteristics: Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.

Purification: Antigen Immunoaffiinity Purification

Immunogen: Rabbits were immunized with a synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1

Isotype: IgG

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 μg/mL). Antibody shows some cross reactivity (~10 % by ELISA) with other phosphoserine containing proteins. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: Sphingosine Kinase 1

UniProt: Q8CI15

Pathways: VEGF Signaling