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Vimentin antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects Vimentin in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Rabbit, Sheep, Horse and Guinea Pig.

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : VIM: Red DAPI:Blue Gene Name : VIM Submitted by : Dr. (Vimentin antibody (N-Term))

IHC

Host: Mouse Target Name: VIM Sample Tissue: Mouse Small Intestine Antibody Dilution: 1ug/ml (Vimentin antibody (N-Term))

submitted by:Carl LundinStanford UniversityThe Vimentin antibody works well for visualization of the vimentin cytoskeleton (see attached images). (Vimentin antibody (N-Term))

IHC

Rabbit Anti-VIM Antibody Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (Vimentin antibody (N-Term))

IHC

5 images

Catalog No. ABIN2773915

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Quick Overview for Vimentin antibody (N-Term) (ABIN2773915)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human, Rat, Mouse, Cow, Dog, Rabbit, Sheep, Horse, Guinea Pig

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product VIM Primary Antibody

Binding Specificity
32

31

17

16

12

12

10

7

6

5

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term

Sequence

LNDRFANYID KVRFLEQQNK ILLAELEQLK GQGKSRLGDL YEEEMRELRR

Predicted Reactivity

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics

This is a rabbit polyclonal antibody against VIM. It was validated on Western Blot using a cell lysate as a positive control.

Purification

Affinity Purified

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human VIM
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated

Application Notes

Optimal working dilutions should be determined experimentally by the investigator.

Comment

Antigen size: 466 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

-20 °C

Storage Comment

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

VIM

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ36605
Protein Interaction Partner: PKN1, PPL, PLA2G2A, PA2G4, KRT20, TXLNB, CWF19L2, PNMA5, UBC, TP53, CHFR, SPRTN, SUMO2, SUMO3, TRIM68, STAU1, ASB15, ASB9, ASB16, ASB2, RNF2, EEF1D, SERPINH1, COPS8, FOXK1, YWHAQ, PARK2, FBXO6, CASP7, CASP3, KAT7, UBD, PINK1, BAG3, GSK3B, PAN2, CAPN1, SVI
Protein Size: 466

Molecular Weight

54 kDa

Gene ID

7431

NCBI Accession

NM_003380, NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis