PPP1R12A antibody (C-Term)

Catalog No. ABIN2774306

The Rabbit Polyclonal anti-PPP1R12A antibody has been validated for WB. It is suitable to detect PPP1R12A in samples from Human, Mouse, Rat, Rabbit, Cow, Dog, Horse and Guinea Pig.

Quick Overview for PPP1R12A antibody (C-Term) (ABIN2774306)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP1R12A Antibody

Binding Specificity: C-Term

Sequence: QDSDENEQEQ QSDTEEGSNK KETQTDSISR YETSSTSAGD RYDSLLGRSG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PPP1R12A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PPP1R12A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1030 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A

Background: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase.
Alias Symbols: MBS, MGC133042, MYPT1, M130
Protein Interaction Partner: TP53, UBC, NUAK1, RPA3, RPA2, RPA1, TRIM47, AARSD1, PUS1, P3H1, RPRD1B, XPO5, OSGEP, NSUN2, NUSAP1, TTLL12, NUDT5, ARPC2, ACTR3, CUL1, TPP2, PFDN5, PFDN1, NRD1, PAN2, LATS1, gag, FMNL1, PAXIP1, IDH3G, CDV3, RHOA, LRRK2, ATXN10, SIAH2, FBXO25, ARRB2, ELAVL
Protein Size: 1030

Molecular Weight: 113 kDa

Gene ID: 4659

NCBI Accession: NM_002480, NP_002471

UniProt: O14974

Pathways: M Phase