PPP1R12A antibody (Myosin Phosphatase, Target Subunit 1) (C-Term)

Details for Product anti-PPP1R12A Antibody No. ABIN2774306
Antigen
Epitope
C-Term
33
25
15
13
9
8
8
8
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
174
137
116
10
9
9
5
4
2
2
2
2
2
1
Host
Rabbit
172
1
1
Clonality
Polyclonal
Conjugate
This PPP1R12A antibody is un-conjugated
9
9
9
9
9
9
9
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
106
53
52
28
21
9
8
2
2
2
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human PPP1R12A
Blocking Peptide Blocking peptide for this product available: ABIN982097
Sequence QDSDENEQEQ QSDTEEGSNK KETQTDSISR YETSSTSAGD RYDSLLGRSG
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against PPP1R12A. It was validated on Western Blot.
Purification Affinity Purified
Antigen
Alternative Name PPP1R12A (PPP1R12A Antibody Abstract)
Background Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase.
Alias Symbols: MBS, MGC133042, MYPT1, M130
Protein Interaction Partner: TP53, UBC, NUAK1, RPA3, RPA2, RPA1, TRIM47, AARSD1, PUS1, P3H1, RPRD1B, XPO5, OSGEP, NSUN2, NUSAP1, TTLL12, NUDT5, ARPC2, ACTR3, CUL1, TPP2, PFDN5, PFDN1, NRD1, PAN2, LATS1, gag, FMNL1, PAXIP1, IDH3G, CDV3, RHOA, LRRK2, ATXN10, SIAH2, FBXO25, ARRB2, ELAVL
Protein Size: 1030
Molecular Weight 113 kDa
Gene ID 4659
NCBI Accession NM_002480, NP_002471
UniProt O14974
Pathways M Phase
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1030 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN2774306) anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (Image 1)
Image no. 2 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN2774306) WB Suggested Anti-PPP1R12A AntibodyTitration: 1.0 µg/mL Positive Control: Jurkat Who...