PYCARD antibody (C-Term)

Catalog No. ABIN2774827

The Rabbit Polyclonal anti-PYCARD antibody is suitable to detect PYCARD in samples from Human, Rat, Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for PYCARD antibody (C-Term) (ABIN2774827)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human, Rat, Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PYCARD Antibody

Binding Specificity: C-Term

Purpose: PYCARD Antibody - C-terminal region

Sequence: ALIARVTNVE WLLDALYGKV LTDEQYQAVR AEPTNPSKMR KLFSFTPAWN

Predicted Reactivity: Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against PYCARD. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PYCARD

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36561-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD

Background:

Background Information: Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis.

Gene Name: PYD and CARD domain containing

Alternative Symbols: ASC, TMS, TMS1, CARD5, TMS-1

Protein Name: Apoptosis-associated speck-like protein containing a CARD

Molecular Weight: 21kDa

Gene ID: 29108

NCBI Accession: NP_037390

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome