DDX21 antibody (N-Term)

Catalog No. ABIN2775197

The Rabbit Polyclonal anti-DDX21 antibody has been validated for WB. It is suitable to detect DDX21 in samples from Human, Rat, Horse and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for DDX21 antibody (N-Term) (ABIN2775197)

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Reactivity: Human, Rat, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX21 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX21 Antibody

Binding Specificity: N-Term

Sequence: MPGKLRSDAG LESDTAMKKG ETLRKQTEEK EKKEKPKSDK TEEIAEEEET

Predicted Reactivity: Horse: 79%, Human: 100%, Rat: 91%, Yeast: 83%

Characteristics: This is a rabbit polyclonal antibody against DDX21. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX21

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 783 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX21 antibody (ABIN2775197)

De Wever, Lloyd, Nasa, Nimick, Trinkle-Mulcahy, Gourlay, Morrice, Moorhead: "Isolation of human mitotic protein phosphatase complexes: identification of a complex between protein phosphatase 1 and the RNA helicase Ddx21." in: PLoS ONE, Vol. 7, Issue 6, pp. e39510, (2012) (PubMed).

Naji, Ambrus, Cimerman?i?, Reyes, Johnson, Filbrandt, Huber, Vesely, Krogan, Yates, Saphire, Gerace: "Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 4, pp. M111.015313, (2012) (PubMed).

Target Details for DDX21

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Alternative Name: DDX21

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Alias Symbols: GUA, GURDB, RH-II/GU, RH-II/GuA
Protein Interaction Partner: HUWE1, PA2G4, KPNA3, CEP250, SUMO2, SUMO3, STAU1, IVNS1ABP, UBC, MDM2, LGR4, SUMO1, ERG, WWOX, EED, rev, SRPK2, SRPK3, FBXO6, TARDBP, LYN, AICDA, PAN2, vif, WHSC1, VCAM1, ITGA4, IL7R, FN1, CSNK2A1, YWHAE, SMURF1, H2AFX, APTX, ESR1, FTSJ3, PCDHA2, RRP7A, R
Protein Size: 783

Molecular Weight: 86 kDa

Gene ID: 9188

NCBI Accession: NM_004728, NP_004719

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interactome