UBE2L6 antibody (N-Term)

Catalog No. ABIN2775687

There is 1 publication for this product available. The Rabbit Polyclonal anti-UBE2L6 antibody is suitable to detect UBE2L6 in samples from Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for UBE2L6 antibody (N-Term) (ABIN2775687)

Target: UBE2L6 (Ubiquitin-Conjugating Enzyme E2L 6 (UBE2L6))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2L6 Antibody

Binding Specificity: N-Term

Purpose: UBE2L6 Antibody - N-terminal region

Sequence: LLLPDQPPYH LKAFNLRISF PPEYPFKPPM IKFTTKIYHP NVDENGQICL

Predicted Reactivity: Cow: 85%, Dog: 85%, Guinea Pig: 85%, Horse: 86%, Human: 100%, Mouse: 85%, Pig: 92%, Rabbit: 100%, Rat: 85%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against UBE2L6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2L6

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43033-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2L6 antibody (ABIN2775687)

Li, Wang, Yang, Li, Qin, Dong, Zhu, Liang, Liang, Han: "The Spen homolog Msx2-interacting nuclear target protein interacts with the E2 ubiquitin-conjugating enzyme UbcH8." in: Molecular and cellular biochemistry, Vol. 288, Issue 1-2, pp. 151-7, (2006) (PubMed).

Target Details for UBE2L6

Target: UBE2L6 (Ubiquitin-Conjugating Enzyme E2L 6 (UBE2L6))

Alternative Name: UBE2L6

Background:

Background Information: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L6 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: Ubiquitin-conjugating enzyme E2L 6

Alternative Symbols: RIG-B, UBCH8

Protein Name: Ubiquitin/ISG15-conjugating enzyme E2 L6

Molecular Weight: 18kDa

Gene ID: 9246

NCBI Accession: NP_004214

UniProt: Q8N5D8