EGLN1 antibody (C-Term)

Catalog No. ABIN2775907

The Rabbit Polyclonal anti-EGLN1 antibody is suitable to detect EGLN1 in samples from Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for EGLN1 antibody (C-Term) (ABIN2775907)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGLN1 Antibody

Binding Specificity: C-Term

Purpose: EGLN1 Antibody - C-terminal region

Sequence: QPAYATRYAI TVWYFDADER ARAKVKYLTG EKGVRVELNK PSDSVGKDVF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EGLN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGLN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099492

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background:

Background Information: EGLN1 has a role in the regulation of hypoxia-inducible factor. It is not affected by overexpression or downregulation of HIF-2alpha.

Gene Name: Egl nine homolog 1 (C. elegans)

Alternative Symbols: HPH2, PHD2, SM20, ECYT3, HALAH, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2

Protein Name: Egl nine homolog 1

Molecular Weight: 46kDa

Gene ID: 54583

NCBI Accession: NP_071334

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect