GABRE antibody (Middle Region)

Catalog No. ABIN2776248

This Rabbit Polyclonal antibody specifically detects GABRE in WB. It exhibits reactivity toward Human, Mouse, Guinea Pig, Rabbit and Rat and has been mentioned in 3+ publications.

Quick Overview for GABRE antibody (Middle Region) (ABIN2776248)

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Reactivity: Human, Mouse, Guinea Pig, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRE Antibody

Binding Specificity: Middle Region

Sequence: KNSWKLFQFD FTGVSNKTEI ITTPVGDFMV MTIFFNVSRR FGYVAFQNYV

Predicted Reactivity: Guinea Pig: 79%, Human: 100%, Mouse: 85%, Rabbit: 86%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against GABRE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GABRE

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 393 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GABRE antibody (ABIN2776248)

Fatemi, Folsom, Rooney, Thuras: "Expression of GABAA ?2-, ?1- and ?-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder." in: Translational psychiatry, Vol. 3, pp. e303, (2013) (PubMed).

Hengen, Nelson, Stang, Johnson, Crader, Watters, Mitchell, Behan: "Increased GABA(A) receptor ?-subunit expression on ventral respiratory column neurons protects breathing during pregnancy." in: PLoS ONE, Vol. 7, Issue 1, pp. e30608, (2012) (PubMed).

Hengen, Gomez, Stang, Johnson, Behan: "Changes in ventral respiratory column GABAaR ?- and ?-subunits during hibernation mediate resistance to depression by EtOH and pentobarbital." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 2, pp. R272-83, (2011) (PubMed).

Target Details for GABRE

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Alternative Name: GABRE

Background: GABRE belongs to the ligand-gated ionic channel (TC 1.A.9) family. It encodes the gamma-aminobutyric acid (GABA) A receptor which is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes an epsilon subunit. It is mapped to chromosome Xq28 in a cluster comprised of genes encoding alpha 3, beta 4 and theta subunits of the same receptor. Alternatively spliced transcript variants encoding different isoforms have been identified.
Protein Size: 393

Molecular Weight: 43 kDa

Gene ID: 2564

NCBI Accession: NM_021984, NP_068819

Pathways: Synaptic Membrane