TARDBP antibody (N-Term)

Catalog No. ABIN2776487

This Rabbit Polyclonal antibody specifically detects TARDBP in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Horse, Guinea Pig and Rabbit.

Quick Overview for TARDBP antibody (N-Term) (ABIN2776487)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TARDBP Antibody

Binding Specificity: N-Term

Sequence: VYVVNYPKDN KRKMDETDAS SAVKVKRAVQ KTSDLIVLGL PWKTTEQDLK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against TARDBP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TARDBP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 414 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TDP-43, ALS10
Protein Interaction Partner: ZFAND2B, TARDBP, UBC, SUMO2, STAU1, SUMO3, RNF4, USP8, RBM45, RNF2, LSM6, UBE2E3, GNB2L1, MED6, RPA1, WWOX, RPA3, RPA2, PARK2, EED, BMI1, RBM39, BAG2, RPL23, DDX21, PRPF3, USP10, SART1, RPL14, H1FX, FUBP3, PABPC4, EIF4G3, EIF3I, EIF3H, EIF3C, EIF3B, EIF3A
Protein Size: 414

Molecular Weight: 45 kDa

Gene ID: 23435

NCBI Accession: NM_007375, NP_031401

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion