MUC1 antibody (N-Term)

Catalog No. ABIN2776851

The Rabbit Polyclonal anti-MUC1 antibody is suitable to detect MUC1 in samples from Human, Cow, Dog, Pig, Rabbit and Goat. It has been validated for IHC and WB.

Quick Overview for MUC1 antibody (N-Term) (ABIN2776851)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Cow, Dog, Pig, Rabbit, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-MUC1 Antibody

Binding Specificity: N-Term

Purpose: MUC1 Antibody - N-terminal region

Sequence: SATQRSSVPS STEKNALSTG VSFFFLSFHI SNLQFNSSLE DPSTDYYQEL

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 86%, Human: 100%, Pig: 82%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against MUC1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MUC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41502-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background:

Background Information: MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.

Gene Name: Mucin 1, cell surface associated

Alternative Symbols: EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADTKD2, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC

Protein Name: Mucin-1

Molecular Weight: 27kDa

Gene ID: 4582

NCBI Accession: NP_002447

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling