Leptin antibody (N-Term)

Catalog No. ABIN2776943

This Rabbit Polyclonal antibody specifically detects Leptin in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Goat, Rabbit, Sheep and Guinea Pigand has been mentioned in 1 publication.

Quick Overview for Leptin antibody (N-Term) (ABIN2776943)

Target: Leptin (LEP)

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Leptin Antibody

Binding Specificity: N-Term

Sequence: MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 91%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%, Sheep: 91%

Characteristics: This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LEP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 167 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Leptin antibody (ABIN2776943)

Müller, Kowalewski, Reichler, Kollár, Balogh: "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." in: Reproduction in domestic animals = Zuchthygiene, Vol. 52 Suppl 2, pp. 187-192, (2017) (PubMed).

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: LEP

Background: LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regula
Alias Symbols: OB, OBS
Protein Interaction Partner: Hk3, SIGLEC6, GHRL, LEPR, UCN, PRKAA2, CLU, CRP, A2M, STAT3,
Protein Size: 167

Molecular Weight: 19 kDa

Gene ID: 3952

NCBI Accession: NM_000230, NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process