C4B antibody (N-Term)

Catalog No. ABIN2777022

This Rabbit Polyclonal antibody specifically detects C4B in WB. It exhibits reactivity toward Human, Mouse, Horse, Dog, Guinea Pig, Pig and Rat.

Quick Overview for C4B antibody (N-Term) (ABIN2777022)

Target: C4B (C4b) (Complement Component C4b (C4b))

Reactivity: Human, Mouse, Horse, Dog, Guinea Pig, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C4B Antibody

Binding Specificity: N-Term

Purpose: C4B Antibody - N-terminal region

Sequence: QTDQPIYNPG QRVRYRVFAL DQKMRPSTDT ITVMVENSHG LRVRKKEVYM

Predicted Reactivity: Dog: 92%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against C4B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human C4B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41841-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for C4B

Target: C4B (C4b) (Complement Component C4b (C4b))

Alternative Name: C4B

Background:

Background Information: C4B is the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Complement component 4B, telomeric

Alternative Symbols: C4A, C4F, CO4, C4B1, C4B2, C4B3, C4B5, C4A91, C4B12

Protein Name: C4B1 EMBL AAR89163.1

Molecular Weight: 193kDa

Gene ID: 432395

NCBI Accession: NP_001002029

UniProt: Q6U2E9

Pathways: Complement System