BDNF antibody (Middle Region)

Catalog No. ABIN2777093

This Rabbit Polyclonal antibody specifically detects BDNF in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Pig, Rabbit, Horse and Dog and has been mentioned in 6+ publications.

Quick Overview for BDNF antibody (Middle Region) (ABIN2777093)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BDNF Antibody

Binding Specificity: Middle Region

Sequence: EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG

Predicted Reactivity: Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BDNF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BDNF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 247 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BDNF antibody (ABIN2777093)

McCarthy, Mueller, Cannon, Huizenga, Darnell, Bhide, Sadri-Vakili: "Prenatal Cocaine Exposure Alters BDNF-TrkB Signaling in the Embryonic and Adult Brain." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 365-374, (2017) (PubMed).

McCarthy, Bell, Cannon, Mueller, Huizenga, Sadri-Vakili, Fadool, Bhide: "Reversal Learning Deficits Associated with Increased Frontal Cortical Brain-Derived Neurotrophic Factor Tyrosine Kinase B Signaling in a Prenatal Cocaine Exposure Mouse Model." in: Developmental neuroscience, Vol. 38, Issue 5, pp. 354-364, (2016) (PubMed).

Kight, McCarthy: "Sex differences and estrogen regulation of BDNF gene expression, but not propeptide content, in the developing hippocampus." in: Journal of neuroscience research, Vol. 95, Issue 1-2, pp. 345-354, (2016) (PubMed).

Kasemeier-Kulesa, Morrison, Lefcort, Kulesa: "TrkB/BDNF signalling patterns the sympathetic nervous system." in: Nature communications, Vol. 6, pp. 8281, (2015) (PubMed).

Vassoler, White, Schmidt, Sadri-Vakili, Pierce: "Epigenetic inheritance of a cocaine-resistance phenotype." in: Nature neuroscience, Vol. 16, Issue 1, pp. 42-7, (2012) (PubMed).

McCarthy, Zhang, Darnell, Sangrey, Yanagawa, Sadri-Vakili, Bhide: "Cocaine alters BDNF expression and neuronal migration in the embryonic mouse forebrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 38, pp. 13400-11, (2011) (PubMed).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Alias Symbols: MGC34632
Protein Interaction Partner: UBC, AGO3, INPP5K, F11R, JUNB, APP, ELAVL1, Ntrk2, CADPS2, MBTPS1, SORT1, NOS3, NTF4, NTF3, ESR1, NCAM1, CPE, BDNF,
Protein Size: 247

Molecular Weight: 27 kDa

Gene ID: 627

NCBI Accession: NM_001709, NP_001700

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity