MGMT antibody (Middle Region)

Catalog No. ABIN2777118

The Rabbit Polyclonal anti-MGMT antibody (ABIN2777118) specifically detects MGMT in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae samples.

Quick Overview for MGMT antibody (Middle Region) (ABIN2777118)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MGMT Antibody

Binding Specificity: Middle Region

Sequence: ISYQQLAALA GNPKAARAVG GAMRGNPVPI LIPCHRVVCS SGAVGNYSGG

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%

Characteristics: This is a rabbit polyclonal antibody against MGMT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Alias Symbols: -
Protein Interaction Partner: UBC, BRCA2, ANXA2, ANXA1, SLC25A6, SLC25A4, ACTB, CCNB1, FBL, EEF1B2, EEF1A2, EEF1A1, DDX5, CDKN1A, CDK2, CDK1, H2BFM, FBXO27, TUBA1C, ANP32E, IL25, DDX24, CDK12, FBXO2, USP19, TUBB4B, PRPF4, RNF8, EIF3H, CDK10, HIST1H4A, HIST1H2AM, HIST3H3, SMC1A, YWHAZ,
Protein Size: 207

Molecular Weight: 22 kDa

Gene ID: 4255

NCBI Accession: NM_002412, NP_002403

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus