MGMT antibody (Middle Region)

Catalog No. ABIN2777118

This anti-MGMT antibody is a Rabbit Polyclonal antibody detecting MGMT in WB. Suitable for Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae.

Quick Overview for MGMT antibody (Middle Region) (ABIN2777118)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MGMT Antibody

Binding Specificity: Middle Region

Sequence: ISYQQLAALA GNPKAARAVG GAMRGNPVPI LIPCHRVVCS SGAVGNYSGG

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%

Characteristics: This is a rabbit polyclonal antibody against MGMT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Alias Symbols: -
Protein Interaction Partner: UBC, BRCA2, ANXA2, ANXA1, SLC25A6, SLC25A4, ACTB, CCNB1, FBL, EEF1B2, EEF1A2, EEF1A1, DDX5, CDKN1A, CDK2, CDK1, H2BFM, FBXO27, TUBA1C, ANP32E, IL25, DDX24, CDK12, FBXO2, USP19, TUBB4B, PRPF4, RNF8, EIF3H, CDK10, HIST1H4A, HIST1H2AM, HIST3H3, SMC1A, YWHAZ,
Protein Size: 207

Molecular Weight: 22 kDa

Gene ID: 4255

NCBI Accession: NM_002412, NP_002403

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus