Androgen Receptor antibody (N-Term)

Catalog No. ABIN2777192

The Rabbit Polyclonal anti-Androgen Receptor antibody has been validated for WB. It is suitable to detect Androgen Receptor in samples from Human, Mouse, Rat, Pig, Dog, Cow, Horse, Rabbit, Guinea Pig and Sheep.

Quick Overview for Androgen Receptor antibody (N-Term) (ABIN2777192)

Target: Androgen Receptor (AR)

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Horse, Rabbit, Guinea Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Androgen Receptor Antibody

Binding Specificity: N-Term

Sequence: MEVQLGLGRV YPRPPSKTYR GAFQNLFQSV REVIQNPGPR HPEAASAAPP

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against AR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 920 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: AR

Background: AR has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS).The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
Alias Symbols: AIS, DHTR, HUMARA, KD, NR3C4, SBMA, SMAX1, TFM, HYSP1
Protein Interaction Partner: AATF, SPOP, UBC, TSG101, TAF1, EP300, DAPK3, USP12, TTC5, SIRT1, RNF6, MAGEA11, VCP, KIF1A, NSD1, WIPI1, NDE1, NCOA1, REL, SMAD1, WDR77, AR, RANBP9, VHL, SKP2, RNF14, EZH2, BMI1, DCAF6, SUZ12, EED, RNF2, RBL1, PTGES3, NCOA4, SUMO2, CUL2, UXT, TCF4, ACTB,
Protein Size: 920

Molecular Weight: 99 kDa

Gene ID: 367

NCBI Accession: NM_000044, NP_000035

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding