RACGAP1 antibody (N-Term)

Catalog No. ABIN2777488

The Rabbit Polyclonal anti-RACGAP1 antibody (ABIN2777488) specifically detects RACGAP1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit and Dog samples.

Quick Overview for RACGAP1 antibody (N-Term) (ABIN2777488)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RACGAP1 Antibody

Binding Specificity: N-Term

Purpose: RACGAP1 Antibody - N-terminal region

Sequence: EILSEGNEVQ FIQLAKDFED FRKKWQRTDH ELGKYKDLLM KAETERSALD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RACGAP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RACGAP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109897

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: RACGAP1

Background:

Background Information: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.

Gene Name: Rac GTPase activating protein 1

Alternative Symbols: CYK4, ID-GAP, HsCYK-4, MgcRacGAP

Protein Name: Rac GTPase-activating protein 1

Molecular Weight: 71kDa

Gene ID: 29127

NCBI Accession: NP_037409

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor