PPARG antibody (N-Term)

Catalog No. ABIN2777645

The Rabbit Polyclonal anti-PPARG antibody is suitable to detect PPARG in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Sheep, Guinea Pig, Goat and Horse. It has been validated for WB and IHC.

Quick Overview for PPARG antibody (N-Term) (ABIN2777645)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Sheep, Guinea Pig, Goat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPARG Antibody

Binding Specificity: N-Term

Sequence: SHSFDIKPFT TVDFSSISTP HYEDIPFTRT DPVVADYKYD LKLQEYQSAI

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against PPARG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPARG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 505 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARG

Background: PPARG is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.
Alias Symbols: GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma
Protein Interaction Partner: HDAC3, NCOA1, NCOA3, SUMO1, UBC, SUMO2, RXRA, MED1, HMGA1, EP300, NCOA2, CDC34, PRKAA2, PRKAA1, PPARGC1A, ASH2L, RBBP5, NSD1, NCOA6, PAXIP1, MUC1, NR3C1, SMAD2, CREBBP, ALOX15B, ZFYVE9, USP7, NCOR1, CDK5, SMARCD3, SMARCA4, NEDD4, BCAS2, MIF4GD, MED14, JAZ
Protein Size: 505

Molecular Weight: 56 kDa

Gene ID: 5468

NCBI Accession: NM_015869, NP_056953

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation