Retinoblastoma 1 antibody (C-Term)

Catalog No. ABIN2777807

This Rabbit Polyclonal antibody specifically detects Retinoblastoma 1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for Retinoblastoma 1 antibody (C-Term) (ABIN2777807)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: C-Term

Sequence: IMMCSMYGIC KVKNIDLKFK IIVTAYKDLP HAVQETFKRV LIKEEEYDSI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 928 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1

Background: Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.
Alias Symbols: RB, pRb, OSRC, pp110, p105-Rb
Protein Interaction Partner: RAF1, E2F1, ABL1, HDAC1, KDM2A, SMARCA4, USP7, UBC, DYRK1A, NEFM, DYRK1B, MAGEA11, ENC1, PPP1CA, CDKN1C, CDKN1A, CDK6, CDK4, CDK2, CCNE1, CCNA2, CCND1, HDAC11, PAX5, HIF1A, E2F4, CEBPE, CCNA1, SUMO1, UBE2I, TFAP2A, CDK3, CDK1, CCNC, CCNB1, MRPS18B, SUMO2,
Protein Size: 928

Molecular Weight: 106 kDa

Gene ID: 5925

NCBI Accession: NM_000321, NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy