NCOA1 antibody (Middle Region)

Catalog No. ABIN2778219

This Rabbit Polyclonal antibody specifically detects NCOA1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbit.

Quick Overview for NCOA1 antibody (Middle Region) (ABIN2778219)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCOA1 Antibody

Binding Specificity: Middle Region

Sequence: GLLHNNDRLS DGDSKYSQTS HKLVQLLTTT AEQQLRHADI DTSCKDVLSC

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NCOA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NCOA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1399 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: NCOA1

Background: NCOA1 acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. NCOA1 binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion.The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: F-SRC-1, KAT13A, MGC129719, MGC129720, NCoA-1, RIP160, SRC1, bHLHe42, bHLHe74
Protein Interaction Partner: ASCC1, NR1I2, PPARG, AR, VDR, THRB, RXRA, RORB, RORA, RARA, HNF4G, NR6A1, NR5A2, ESRRA, ESR1, PAGR1, Esr2, NR3C1, CXADR, PPARA, AHR, KANK2, NCOA2, TRIM24, NCOA1, ANIB1, PGR, COPS5, UBE2L3, TP53, NCOA6, EP300, UBC, RARB, PPARD, RARG, RBBP6, CREBBP, STAT3,
Protein Size: 1399

Molecular Weight: 152 kDa

Gene ID: 8648

NCBI Accession: NM_147223, NP_671756

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha