GLIS3 antibody (N-Term)

Catalog No. ABIN183860

The Rabbit Polyclonal anti-GLIS3 antibody is suitable to detect GLIS3 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for GLIS3 antibody (N-Term) (ABIN183860)

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLIS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLIS3 Antibody

Binding Specificity: N-Term

Purpose: GLIS3 Antibody - N-terminal region

Sequence: PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE SLASTTLSLT

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GLIS3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLIS3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39924-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLIS3

Target: GLIS3 (GLIS Family Zinc Finger 3 (GLIS3))

Alternative Name: GLIS3

Background:

Background Information: GLIS3 is a member of the GLI-similar zinc finger protein family and has five C2H2-type zinc finger domains. GLIS3 functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH).

Gene Name: GLIS family zinc finger 3

Alternative Symbols: NDH, ZNF515

Protein Name: Zinc finger protein GLIS3

Molecular Weight: 84kDa

Gene ID: 169792

NCBI Accession: NP_689842

UniProt: Q8NEA6