EYA4 antibody (N-Term)

Catalog No. ABIN2778551

The Rabbit Polyclonal anti-EYA4 antibody (ABIN2778551) specifically detects EYA4 in WB. The antibody is reactive with Human, Rabbit, Guinea Pig, Mouse, Dog and Zebrafish (Danio rerio) samples.

Quick Overview for EYA4 antibody (N-Term) (ABIN2778551)

Target: EYA4 (Eyes Absent Homolog 4 (EYA4))

Reactivity: Human, Rabbit, Guinea Pig, Mouse, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EYA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EYA4 Antibody

Binding Specificity: N-Term

Purpose: Eya4 Antibody - N-terminal region

Sequence: MEDTQDLNEQ SVKKTCPEAD VSEPQNSRSM EMQDLASPHA LVGGSDTPGS

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against Eya4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Eya4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39975-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EYA4

Target: EYA4 (Eyes Absent Homolog 4 (EYA4))

Alternative Name: Eya4

Background:

Background Information: Eya4 is a tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Eya4 promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. It may be involved in development of the eye.

Gene Name: Eyes absent 4 homolog (Drosophila)

Alternative Symbols: B130023L16Rik

Protein Name: Eyes absent homolog 4

Molecular Weight: 67kDa

Gene ID: 14051

NCBI Accession: NP_034297

UniProt: Q9Z191

Pathways: Sensory Perception of Sound