INSIG1 antibody (Middle Region)

Catalog No. ABIN2778635

The Rabbit Polyclonal anti-INSIG1 antibody (ABIN2778635) specifically detects INSIG1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for INSIG1 antibody (Middle Region) (ABIN2778635)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INSIG1 Antibody

Binding Specificity: Middle Region

Purpose: INSIG1 Antibody - middle region

Sequence: TTWLFHKTRS NYRVFLKSPI VIESSKPPIL RARKILEENL TVDYDKDYLF

Predicted Reactivity: Human: 100%, Mouse: 82%

Characteristics: This is a rabbit polyclonal antibody against INSIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40161-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1

Background:

Background Information: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: Insulin induced gene 1

Alternative Symbols: CL6

Protein Name: Insulin induced gene 1 EMBL EAL23728.1

Molecular Weight: 36kDa

Gene ID: 3638

NCBI Accession: NP_938150

UniProt: A4D2M9

Pathways: ER-Nucleus Signaling