INSIG1 antibody (Middle Region)

Catalog No. ABIN2778635

The Rabbit Polyclonal anti-INSIG1 antibody (ABIN2778635) specifically detects INSIG1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for INSIG1 antibody (Middle Region) (ABIN2778635)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INSIG1 Antibody

Binding Specificity: Middle Region

Sequence: TTWLFHKTRS NYRVFLKSPI VIESSKPPIL RARKILEENL TVDYDKDYLF

Predicted Reactivity: Human: 100%, Mouse: 82%

Characteristics: This is a rabbit polyclonal antibody against INSIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 330 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1

Background: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: CL-6, MGC1405, CL6
Protein Interaction Partner: UBC, HMGCR, FAF2, SCAP, ERLIN2, ERLIN1, VCP, AMFR, PGRMC1, POR, SREBF1, ELAVL1, RNF139, INSIG2, PSMC3, PSMA7, INSIG1, VAPA, VAPB, SREBF2,
Protein Size: 330

Molecular Weight: 36 kDa

Gene ID: 3638

NCBI Accession: NM_198336, NP_938150

UniProt: A4D2M9

Pathways: ER-Nucleus Signaling