INSIG2 antibody (N-Term)

Catalog No. ABIN2778637

Cited in 1 publication. The Rabbit Polyclonal anti-INSIG2 antibody (ABIN2778637) specifically detects INSIG2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Dog, Pig and Sheep samples.

Quick Overview for INSIG2 antibody (N-Term) (ABIN2778637)

Target: INSIG2 (Insulin Induced Gene 2 (INSIG2))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Dog, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INSIG2 Antibody

Binding Specificity: N-Term

Purpose: INSIG2 Antibody - N-terminal region

Sequence: MAEGETESPG PKKCGPYISS VTSQSVNLMI RGVVLFFIGV FLALVLNLLQ

Predicted Reactivity: Cow: 100%, Dog: 92%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 100%, Rat: 92%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against INSIG2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human INSIG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40165-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-INSIG2 antibody (ABIN2778637)

Yu, Wang, Fok, Coles, Salmon, Pérez: "Rapamycin and dietary restriction induce metabolically distinctive changes in mouse liver." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 70, Issue 4, pp. 410-20, (2015) (PubMed).

Target Details for INSIG2

Target: INSIG2 (Insulin Induced Gene 2 (INSIG2))

Alternative Name: INSIG2

Background:

Background Information: INSIG2 is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Insulin induced gene 2

Alternative Symbols: INSIG-2

Protein Name: Insulin-induced gene 2 protein

Molecular Weight: 25kDa

Gene ID: 51141

NCBI Accession: NP_057217

UniProt: Q9Y5U4

Pathways: ER-Nucleus Signaling