HNRNPD/AUF1 antibody (C-Term)

Catalog No. ABIN2778693

This Rabbit Polyclonal antibody specifically detects HNRNPD/AUF1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Pig and Rabbit and has been mentioned in 3+ publications.

Quick Overview for HNRNPD/AUF1 antibody (C-Term) (ABIN2778693)

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPD/AUF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HNRNPD/AUF1 Antibody

Binding Specificity: C-Term

Sequence: YGYNSQGYGG YGGYDYTGYN NYYGYGDYSN QQSGYGKVSR RGGHQNSYKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HNRPD. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HNRPD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 355 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HNRNPD/AUF1 antibody (ABIN2778693)

Subramaniam, Ooi, Hui: "Transcriptional down-regulation of IGFBP-3 in human hepatocellular carcinoma cells is mediated by the binding of TIA-1 to its AT-rich element in the 3'-untranslated region." in: Cancer letters, Vol. 297, Issue 2, pp. 259-68, (2010) (PubMed).

Kang, Chen, Kim, Kang, Park: "Regulation of the hTERT promoter activity by MSH2, the hnRNPs K and D, and GRHL2 in human oral squamous cell carcinoma cells." in: Oncogene, Vol. 28, Issue 4, pp. 565-74, (2009) (PubMed).

Dhakras, Hajarnis, Taylor, Curthoys: "cAMP-dependent stabilization of phosphoenolpyruvate carboxykinase mRNA in LLC-PK1-F+ kidney cells." in: American journal of physiology. Renal physiology, Vol. 290, Issue 2, pp. F313-8, (2006) (PubMed).

Target Details for HNRNPD/AUF1

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Alternative Name: HNRPD

Background: HNRPD belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Alias Symbols: P37, AUF1, AUF1A, HNRPD, hnRNPD0
Protein Interaction Partner: NCL, FUS, KAT2B, SUMO2, SUMO3, IVNS1ABP, STAU1, UBC, NEDD8, MDM2, ERG, RPA3, RPA2, RPA1, ASB2, EED, rev, ERI1, RPS12, PTBP1, PAFAH1B3, WNK1, IPO11, EIF3K, KHSRP, DENR, PARK2, DGCR2, GTF3C3, PDLIM7, UQCRC1, HIRA, SNAPC4, SLC3A2, SDF2, COG7, TMEM261, ABCC10
Protein Size: 355

Molecular Weight: 39 kDa

Gene ID: 3184

NCBI Accession: NM_031370, NP_112738

UniProt: Q14103