PPIE antibody (Middle Region)

Catalog No. ABIN2778968

The Rabbit Polyclonal anti-PPIE antibody (ABIN2778968) specifically detects PPIE in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Saccharomyces cerevisiae, Cow, Horse, Pig, Rabbit and Sheep samples.

Quick Overview for PPIE antibody (Middle Region) (ABIN2778968)

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Saccharomyces cerevisiae, Cow, Horse, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPIE Antibody

Binding Specificity: Middle Region

Purpose: PPIE Antibody - middle region

Sequence: KKFSGKTLEE NKEEEGSEPP KAETQEGEPI AKKARSNPQV YMDIKIGNKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 100%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against PPIE. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPIE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107826

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPIE

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Alternative Name: PPIE

Background:

Background Information: PPIE is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: Peptidylprolyl isomerase E (cyclophilin E)

Alternative Symbols: CYP33, CYP-33

Protein Name: Peptidyl-prolyl cis-trans isomerase E

Molecular Weight: 33kDa

Gene ID: 10450

NCBI Accession: NP_006103

UniProt: Q9UNP9